Sputum test for tuberculosis (microscopy and rapid PCR)
TB sputum test · sputum test · AFB smear · TB PCR · rapid TB test
This test checks the phlegm you cough up from your lungs. It looks for the tuberculosis germ.
For a cough lasting more than two or three weeks, coughing up blood, night sweats, weight loss, after contact with someone with TB, and to check treatment.
⏱ Duration: collection takes a few minutes; the rapid result usually comes in 1 to 2 days, a culture can take up to several weeks

Be sure to tell your doctor
How to prepare
- Morning. It is best to collect phlegm in the morning, right after waking up, before eating.
- Mouth. Before collecting, brush your teeth and rinse your mouth with boiled water.
- Samples. Usually 2 to 3 samples are needed, on different days, or in the morning and later in the day. Your doctor will say how many.
- Place. Collect phlegm outdoors or by an open window, away from other people.
What to bring
- The sputum container
- Your referral
- Your ID
- A face mask
How it goes
- Take a sterile container. Open the lid only right before collecting
- Take a few deep breaths and hold your breath
- Cough hard from deep in your chest
- Spit the phlegm into the container. It must be phlegm, not saliva
- Close the lid tightly and take it to the lab
What you'll feel
- Coughing can be tiring
- If the phlegm will not come up, a warm drink the evening before helps
Afterwards
- Until you get the result, wear a mask around other people
- Air out the room
- If the test is positive, your doctor will explain the treatment in detail
Contact a doctor straight away if
- Coughing up a lot of blood: call emergency services
- Severe shortness of breath or chest pain: call emergency services
- A fever that does not go down for several days: see a doctor
Common questions
Can I give saliva if there is no phlegm?
No, saliva will not work. Tell the nurse. There are ways to help you cough it up.
Can tuberculosis be cured?
Yes. Treatment is long, but if you take your medicines regularly, TB is usually cured.