Sperm DNA fragmentation

sperm DNA fragmentation test · DNA fragmentation index · DFI · sperm DNA damage test

This test shows what share of sperm have damaged DNA, the genetic material. This can make it harder to conceive and for an embryo to develop.

For infertility with a normal semen analysis, repeated miscarriages in the partner, failed IVF, and varicocele, which is swollen veins in the scrotum.

⏱ Duration: giving the sample usually takes 10 to 20 minutes; results usually come in a few days

Be sure to tell your doctor

If you had an illness with fever in the last 2 to 3 months
If you smoke, and how often you drink
If you work in heat or with harmful substances
All the medicines you take

How to prepare

Abstinence. Usually 2 to 7 days without ejaculation. The lab will tell you the exact time.
Before the test. For several days: no alcohol, steam baths, sauna, or overheating.
Medicines. Do not stop anything on your own. Tell your doctor what you take.

What to bring

Your referral
Your ID
Past semen analysis results

How it goes

You get a sterile container and are shown to a private room
Wash your hands and penis with soap
Collect all of the semen into the container by masturbating
Close the container and hand it to the staff
The sample is processed in the lab

What you'll feel

Nothing unpleasant physically
It may feel awkward. That is normal

Afterwards

Results usually come in a few days
If fragmentation is high, the doctor will suggest lifestyle changes and any treatment needed
Sperm renew in about 2 to 3 months. Then the test can be repeated

Common questions

Can DNA fragmentation be lowered?

Often, yes. Quitting smoking, less alcohol and overheating, and treating the cause help. Your doctor will make a plan.

Why can the result change?

It is affected by illnesses with fever, stress, and habits over the past few months.